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Stainless Steel Centrifugal Spray Dryer - Ollital. Lab PLC Control System Mini Stainless Steel Spray Dryer Model OLT-SD8000B Max capacity 1500-2000ml/h Temperature range of Inlet air 30C -280C Temperature range of outlet air 40C -120C Precision of temperature ± 1 C Dryer time 1.0-1.5 S Speed of squirmy pump 30-2000ml/h, Consume of dryer air &1.
Get Price2013-6-14u2002·u2002Introduction. Following fertilization, the embryo remains in a state of transcriptional quiescence that is maintained until a species-specific stage (8–16 cell stage in the cow, 2-cell stage in the mouse, 4-cell stage in the pig and 4–8 cell stage in the human) when transcription is resumed through a process referred to as embryonic genome activation .
Get Price2016-11-14u2002·u2002Injected embryos were cultured in M16 medium (Sigma) at 37 °C under 5% CO 2, until transfer at the one-cell stage the same day or at the two-cell stage the following day to …
Get Price2016-12-10u2002·u2002Objective To examine the maturational competence, embryo development and expression of genes involved in oocyte maturation and cumulus expansion (GDF9, BMP15, HAS2, TNFAIP6, FGF17 and FSHr) following two standard methods of bovine COCs vitrification. Methods Bovine cumulus-oocyte complexes (COCs) were aspirated from slaughtered ovaries and then distributed into three groups: …
Get Pricesheep was obtained using an ear puncher for fibroblast culture. The Mongolian sheep adult fibroblasts (SAFs) were cultured using the tissue adherent culturing method and enzyme digestion, and cryopreserved for >10 years in liquid nitrogen. To recover the cells, the frozen vials were removed from the liquid nitrogen and rapidly thawed in a water ...
Get Price2019-11-22u2002·u2002The sheep is an economically important animal, and there is currently a major focus on improving its meat quality through breeding. There are variations in the growth regulation mechanisms of different sheep breeds, making fundamental research on skeletal muscle growth essential in understanding the regulation of (thus far) unknown genes.
Get Price2016-9-7u2002·u2002Somatic embryo (SE) regeneration is an ideal experimental system to realize rapid propagation of excellent clones and genetic improvement for perennial gymnosperms. In the present study, genes encoding the miRNA166 precursor were identified and LamiR166a was successfully transformed into the gymnosperm Larix leptolepis (L. leptolepis) and five LamiR166a over-expressed embryonic cell …
Get PricePublisher of over 50 scientific journals across the life, physical, earth, and health sciences, both independently and in partnership with scientific societies including Cell, Neuron, Immunity, Current Biology, AJHG, and the Trends Journals.
Get PriceJournal of Cell and Animal Biology. October 29, 2020 ·. Characterization and anti-tumor effect of shark liver extraction. In this study, four different extraction methods of shark liver oil were tried, the effect of the solid-liquid ratio, quality of the enzyme, reaction pH and temperature on SLO extraction rate have been discussed. See ...
Get PriceGFP-gRNA was designed to target the coding sequence after the start codon of the GFP gene. A total of 300 pg of the linearized Tol2-CMV:GFP plasmid was injected into the animal pole of 1-cell stage embryos, accompanied by 150 pg of GFP-gRNA and different doses of the Cas9 protein (150 pg, 300 pg, 600 pg or 1200 pg, 300 embryos for each dosage).). The agarose gel …
Get Price2021-9-19u2002·u2002Fetal bovine serum (FBS) is the liquid fraction of clotted blood from fetal calves, depleted of cells, fibrin and clotting factors, but containing a large number of nutritional and macromolecular factors essential for cell growth. Bovine serum albumin is the major component of FBS. Growth factors in FBS are essential for the maintenance and ...
Get Price2019-8-14u2002·u2002Dickkopf-related protein 1 is a progestomedin acting on the bovine embryo during the morula-to-blastocyst transition to program trophoblast elongation
Get PriceSTEMCELL Technologies develops cell culture media, cell separation systems, instruments and other reagents for use in life sciences research.
Get Price2015-10-16u2002·u2002Tan sheep exhibited the smallest body size with 61.54 ± 6.42 (P < 1.0*10 −7, Fig. 1b ). We randomly selected two female sheep each from UQ, Tan, StH and Hu, along with two male sheep from UQ in ...
Get PriceStem Cell 100® rejuvenates adult stem cells and their micro-environments. Stem Cell 100+® is an advanced version of Stem Cell 100® which acts on even more pathways of natural healing and anti-aging. Developed by experts in the anti-aging field, patent-pending Stem Cell 100® is the only supplement proven to double maximum lifespan of an ...
Get PriceAustralia & UK: DDX5 promotes maintenance and function of spematogonia. Gene expression analysis on Mic qPCR Cycler and immunochemistry methods shows that DDX5 regulates appropriate splicing of key genes necessary for spermatogenesis and regulates expression of cell cycle genes in undifferentiated spermatogonia post-transcriptionally.
Get Priceseaweed extract treatment, and the fatty acid content in the olive oil was significantly enri ched in linolenic and oleic acids while palmitoleic, stearic, and linoleic acids were decreased.
Get PriceFood Ingredients & Food Science - Additives, Flavours, Starch. Our mission is to provide and share B2B knowledge enabling people to succeed in business. We accomplish this by providing high-value information and research, robust online and print journalism, innovative exhibitions and conferences, and revered awards. william-reed.com.
Get PriceIncludes the total amounts of digestible protein, nitrogen-free extract, fiber, and fat (multiplied by 2.25), all summed together. Vitamin An organic catalyst, or a component thereof, that facilitates specific and necessary functions.
Get PriceStainless Steel Centrifugal Spray Dryer - Ollital. Lab PLC Control System Mini Stainless Steel Spray Dryer Model OLT-SD8000B Max capacity 1500-2000ml/h Temperature range of Inlet air 30C -280C Temperature range of outlet air 40C -120C Precision of temperature ± 1 C Dryer time 1.0-1.5 S Speed of squirmy pump 30-2000ml/h, Consume of dryer air &1.
Get Price2013-6-14u2002·u2002Introduction. Following fertilization, the embryo remains in a state of transcriptional quiescence that is maintained until a species-specific stage (8–16 cell stage in the cow, 2-cell stage in the mouse, 4-cell stage in the pig and 4–8 cell stage in the human) when transcription is resumed through a process referred to as embryonic genome activation .
Get Price2016-11-14u2002·u2002Injected embryos were cultured in M16 medium (Sigma) at 37 °C under 5% CO 2, until transfer at the one-cell stage the same day or at the two-cell stage the following day to …
Get Price2016-12-10u2002·u2002Objective To examine the maturational competence, embryo development and expression of genes involved in oocyte maturation and cumulus expansion (GDF9, BMP15, HAS2, TNFAIP6, FGF17 and FSHr) following two standard methods of bovine COCs vitrification. Methods Bovine cumulus-oocyte complexes (COCs) were aspirated from slaughtered ovaries and then distributed into three groups: …
Get Pricesheep was obtained using an ear puncher for fibroblast culture. The Mongolian sheep adult fibroblasts (SAFs) were cultured using the tissue adherent culturing method and enzyme digestion, and cryopreserved for >10 years in liquid nitrogen. To recover the cells, the frozen vials were removed from the liquid nitrogen and rapidly thawed in a water ...
Get Price2019-11-22u2002·u2002The sheep is an economically important animal, and there is currently a major focus on improving its meat quality through breeding. There are variations in the growth regulation mechanisms of different sheep breeds, making fundamental research on skeletal muscle growth essential in understanding the regulation of (thus far) unknown genes.
Get Price2016-9-7u2002·u2002Somatic embryo (SE) regeneration is an ideal experimental system to realize rapid propagation of excellent clones and genetic improvement for perennial gymnosperms. In the present study, genes encoding the miRNA166 precursor were identified and LamiR166a was successfully transformed into the gymnosperm Larix leptolepis (L. leptolepis) and five LamiR166a over-expressed embryonic cell …
Get PricePublisher of over 50 scientific journals across the life, physical, earth, and health sciences, both independently and in partnership with scientific societies including Cell, Neuron, Immunity, Current Biology, AJHG, and the Trends Journals.
Get PriceJournal of Cell and Animal Biology. October 29, 2020 ·. Characterization and anti-tumor effect of shark liver extraction. In this study, four different extraction methods of shark liver oil were tried, the effect of the solid-liquid ratio, quality of the enzyme, reaction pH and temperature on SLO extraction rate have been discussed. See ...
Get Price2021-4-8u2002·u2002In sheep, the uterine luminal (LE), superficial glandular (sGE) and glandular (GE) epithelia are secretory cells that secrete and transport water, amino acids, hexose sugars, ions, growth factors, hormones, enzymes, cytokines, mitogens, and vitamins (collectively referred to as histotroph) into the uterine lumen early in pregnancy [1, 2].Histotroph is also transported into the …
Get Price2021-5-25u2002·u2002The DMF platform has become a very good tool for manipulating trace reagents and target cells in a single droplet and has been widely used in the field of cell manipulation (e.g., cell culture [233-236], cell sorting and concentration [237-240], and single-cell manipulation and analysis [241-244]) in recent years.
Get Price2002-3-1u2002·u2002By preserving gametes, embryos, or somatic cells in liquid nitrogen, the individuals contributing the germ plasm or entire DNA can continue to provide genetic material. The pregnancy rate reported here is similar to that seen with domestic sheep monospecies pregnancies with in vitro-produced embryos [ 9 ].
Get Price2014-7-9u2002·u2002Abstract and Figures. Early in cow embryo development, hepatoma-derived growth factor (HDGF) is detectable in uterine fluid. The origin …
Get Price2020-6-22u2002·u2002The objective of this study was to investigate the metabolic and osmotic effects of different doses of glycerol or a glycerol – propylene glycol mixture in Sarda sheep with the aim to identify those able to beneficially modify ewe's metabolic status without harmful changes in red blood cell (RBC) indices. Thereafter, the selected doses were tested for their effects on ewe's …
Get Price2013-10-24u2002·u2002HiMedia is a trusted name in the field of scientific and laboratory equipment around the globe. We are the supplier and exporter of air purifier systems, air sampler systems, colony counters, electric sterilizer. Disinfectants and sanitizers are chemicals used to rapidly inactivate microorganisms on inanimate surfaces.
Get PriceCells were starved of serum for 24 h as described above and then incubated for 24 h with 20 μ m LPA. BrdU (10 μ m; in situ cell proliferation kit; Roche Diagnostics, Meylan, France) was added to the medium for the final hour of culture. Cells were fixed in cold ethanol for 5 min and treated with 2 n HCl. BrdU-labeled cells revealed by ...
Get Price2021-2-15u2002·u2002Heat stress significantly impairs reproduction of sheep, and under current climatic conditions is a significant risk to the efficiency of the meat and wool production, with the impact increasing as global temperatures rise. Evidence from field studies and studies conducted using environmental chambers demonstrate the effects of hot temperatures (≥ 32 °C) on components …
Get Price2020-1-31u2002·u2002The mechanism underlying the potential risk associated with in vitro fertilization and embryo transfer (IVF‑ET) has been previously investigated but remains to be fully elucidated. As the placenta is a critical organ that sustains and protects the fetus, this is an important area of research. The aim of the present study was to determine the difference in trophoblast cell function in the ...
Get Price2021-9-29u2002·u2002After logging in you can choose to receive a newsletter every day at 9.00 am with the latest articles from your selected journals! 1 feeds 17-02-2021 05:49:58 [email protected] 21 feeds 02-11-2020 14:21:20 dmalaspina. 22 feeds 28-09-2020 17:08:56 ntg. 14 feeds 10-06-2020 14:43:57 dalton.
Get Price2021-6-7u2002·u2002The culture media used throughout the in vitro production (IVP) of bovine embryos remain complex. The serum added to culture media in order to improve embryo development negatively impacts the cryotolerance of blastocysts. Periconceptional prostaglandin E2 (PGE2) signaling is known to exert prosurvival effects on in vitro-generated blastocysts.
Get Price2021-9-19u2002·u2002Fetal bovine serum (FBS) is the liquid fraction of clotted blood from fetal calves, depleted of cells, fibrin and clotting factors, but containing a large number of nutritional and macromolecular factors essential for cell growth. Bovine serum albumin is the major component of FBS. Growth factors in FBS are essential for the maintenance and ...
Get Price2016-12-20u2002·u2002Cloning cattle by somatic cell nuclear transfer (SCNT) is an agriculturally important technology and is also used as a model system for the study of mammalian development. The SCNT process is inefficient, typically yielding fewer than 10% live offspring. The majority of losses are the result of embryonic death, failure of the implantation process, and development of a defective placenta.
Get PriceSTEMCELL Technologies develops cell culture media, cell separation systems, instruments and other reagents for use in life sciences research.
Get Price2010-2-9u2002·u2002Gestational diabetes mellitus (GDM) is a form of diabetes that occurs during pregnancy. GDM is a well known risk factor for foetal overgrowth, termed macrosomia which is influenced by maternal hypergycemia and endocrine status through placental circulation. The study was undertaken to investigate the implication of growth factors and their receptors in GDM and macrosomia, and to …
Get Price2010-3-10u2002·u2002Adiponectin is an adipokine, mainly produced by adipose tissue. It regulates several reproductive processes. The protein expression of the adiponectin system (adiponectin, its receptors, AdipoR1 and AdipoR2 and the APPL1 adaptor) in bovine ovary and its role on ovarian cells and embryo, remain however to be determined. Here, we identified the adiponectin system in bovine ovarian cells …
Get PriceBovine embryos were incubated on a tilting machine, with a cyclic ±10° tilt over 1 min, to move the embryo via gravity. 53 They showed significantly more two-cell embryos developing into eight-cell embryos when cultivated in constricted channels with widths of 150 (37.8 ± 11.0%, mean ± SD) and 160 µm (56.7 ± 13.7%) compared with straight ...
Get Price2021-1-28u2002·u2002Repeat breeding is a critical reproductive disorder in cattle. The problem of repeat breeder cattle remains largely unmanageable due to a lack of informative biomarkers. Here, we utilized metabolomic profiling in an attempt to identify metabolites in the blood plasma and uterine luminal fluids. We collected blood and uterine fluid from repeat breeder and healthy cows on day 7 of the estrous cycle.
Get PriceThe objective of this study was to develop an effective ultra-rapid vitrification method and evaluate its effect on maturation, developmental competence and development-related gene expression in ...
Get Price2009-3-1u2002·u2002Background Cancer patients commonly use dietary supplements to 'boost immune function'. A polysaccharide extract from Grifola frondosa (Maitake extract) showed immunomodulatory effects in preclinical studies and therefore the potential for clinical use. Whether oral administration in human produces measurable immunologic effects, however, is unknown. Methods In a phase I/II dose …
Get PriceStainless Steel Centrifugal Spray Dryer - Ollital. Lab PLC Control System Mini Stainless Steel Spray Dryer Model OLT-SD8000B Max capacity 1500-2000ml/h Temperature range of Inlet air 30C -280C Temperature range of outlet air 40C -120C Precision of temperature ± 1 C Dryer time 1.0-1.5 S Speed of squirmy pump 30-2000ml/h, Consume of dryer air &1.
Get Price2013-6-14u2002·u2002Introduction. Following fertilization, the embryo remains in a state of transcriptional quiescence that is maintained until a species-specific stage (8–16 cell stage in the cow, 2-cell stage in the mouse, 4-cell stage in the pig and 4–8 cell stage in the human) when transcription is resumed through a process referred to as embryonic genome activation .
Get Price2016-11-14u2002·u2002Injected embryos were cultured in M16 medium (Sigma) at 37 °C under 5% CO 2, until transfer at the one-cell stage the same day or at the two-cell stage the following day to …
Get Price2016-12-10u2002·u2002Objective To examine the maturational competence, embryo development and expression of genes involved in oocyte maturation and cumulus expansion (GDF9, BMP15, HAS2, TNFAIP6, FGF17 and FSHr) following two standard methods of bovine COCs vitrification. Methods Bovine cumulus-oocyte complexes (COCs) were aspirated from slaughtered ovaries and then distributed into three groups: …
Get Pricesheep was obtained using an ear puncher for fibroblast culture. The Mongolian sheep adult fibroblasts (SAFs) were cultured using the tissue adherent culturing method and enzyme digestion, and cryopreserved for >10 years in liquid nitrogen. To recover the cells, the frozen vials were removed from the liquid nitrogen and rapidly thawed in a water ...
Get Price2019-11-22u2002·u2002The sheep is an economically important animal, and there is currently a major focus on improving its meat quality through breeding. There are variations in the growth regulation mechanisms of different sheep breeds, making fundamental research on skeletal muscle growth essential in understanding the regulation of (thus far) unknown genes.
Get Price2016-9-7u2002·u2002Somatic embryo (SE) regeneration is an ideal experimental system to realize rapid propagation of excellent clones and genetic improvement for perennial gymnosperms. In the present study, genes encoding the miRNA166 precursor were identified and LamiR166a was successfully transformed into the gymnosperm Larix leptolepis (L. leptolepis) and five LamiR166a over-expressed embryonic cell …
Get PricePublisher of over 50 scientific journals across the life, physical, earth, and health sciences, both independently and in partnership with scientific societies including Cell, Neuron, Immunity, Current Biology, AJHG, and the Trends Journals.
Get PriceJournal of Cell and Animal Biology. October 29, 2020 ·. Characterization and anti-tumor effect of shark liver extraction. In this study, four different extraction methods of shark liver oil were tried, the effect of the solid-liquid ratio, quality of the enzyme, reaction pH and temperature on SLO extraction rate have been discussed. See ...
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