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  • Now, PETA urges govt to not use calf serum in Covaxin ...

    2021-6-17u2002·u2002Covaxin does not contain calf serum, but the serum is used in the labs to boost the growth of Vero cells, both the government and Bharat Biotech clarified

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  • (PDF) A Method for Differentiating Fetal Bovine Serum

    A Method for Differentiating Fetal Bovine Serum f rom Newborn Calf Serum. by adding the mean GGT value of 100 % NBCS (81 4.33 IU /L) and FBS (5 IU/L) and multi plying by the dilution factors ...

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    2004-5-1u2002·u2002N-Propargyl-l(R)-aminoindan, rasagiline, an anti-Parkinson drug, was found to increase the protein and mRNA levels of glial cell line-derived neurotrophic factor (GDNF) in human neuroblastoma SH-SY5Y cells, whereas an analogue without a propargyl residue, aminoindan, did not.GDNF is known to protect dopaminergic neurons in animal and cellular models of Parkinson's disease, and the …

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    2010-6-1u2002·u2002Now, the cultures are kept in Serum Optimem for the entire time of culture, though good replacement for this serum-containing medium is preferred. Other groups have experienced similar difficulties using B27 ( Cressey, 2009 ), and an American group has recently published the recipe for a new supplement, N21, a re-defined and modified ...

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    2014-11-25u2002·u2002Human mesenchymal stem cells (hMSCs) from umbilical cord (UC) blood (UCB) and matrix are tested clinically for a variety of pathologies but in vitro expansion using culture media containing fetal bovine serum (FBS) is essential to achieve appropriate cell numbers for clinical use. Human UCB plasma (hUCBP) can be used as a supplement for hMSCs culture, since UCB is rich in soluble growth ...

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    2019-4-10u2002·u2002Natural surfactant replacement, such as Survanta beractant (modified bovine surfactant 8 mg SP-B/mL) by Abbott Laboratories, Curosurf (porcine surfactant 80 mg/mL) by Chiesi, and Infasurf (calf surfactant), are a few of the FDA-approved preventive medications for infants with ARDS or premature babies at the risk of developing RDS.

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    2018-9-5u2002·u2002The cells were maintained in MEM-FCS (Minimum Essential Medium (MEM; Sigma-Aldrich Corp., USA) supplemented with 10% fetal calf serum (FCS; Gibco-BRL-Life Technologies, UK), 4 mM glutamine (Sigma-Aldrich Corp., USA) and 40 mg ml −1 gentamycin (Chinoin Private Co., Hungary), at 37 °C in 5% CO 2 and 95% air atmosphere. Sub-confluent cultures ...

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    2021-8-26u2002·u2002Presence of serum in the media has many drawbacks and can lead to serious misinterpretations in immunological studies [2, 3].A number of serum-free media have been developed [4, 5].These media are generally specifically formulated to support the culture of a single cell type, such as Knockout Serum Replacement and Knockout DMEM from Thermo Fisher Scientific, and mTESR …

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    2004-5-1u2002·u2002N-Propargyl-l(R)-aminoindan, rasagiline, an anti-Parkinson drug, was found to increase the protein and mRNA levels of glial cell line-derived neurotrophic factor (GDNF) in human neuroblastoma SH-SY5Y cells, whereas an analogue without a propargyl residue, aminoindan, did not.GDNF is known to protect dopaminergic neurons in animal and cellular models of Parkinson's …

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    2017-3-14u2002·u2002HEK293 cells and HEK293-GFP cells (GenTarget, Inc. Cat. No. SC001, San Diego, CA, USA) were cultured in Dulbecco's modified Eagle's medium containing 10% fetal calf serum.

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  • A convenient protocol for establishing a human cell ...

    2018-7-18u2002·u2002The optic nerve is severed at the nerve head to detach the retina. The retinae are pooled in KCl buffer (0.3M KCl, 10mM HEPES, 0.5mM CaCl2, 1mM MgCl2; pH 7.0) with 48% sucrose (w/v) and agitated vigorously for 2 minutes on a rotation mixer after which the solution is centrifuged for 5 minutes at 5,000g.

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  • Co-ultramicronized Palmitoylethanolamide/Luteolin

    2015-11-18u2002·u2002Oligodendrocytes have limited ability to repair the damage to themselves or to other nerve cells, as seen in demyelinating diseases like multiple sclerosis. An important strategy may be to replace ...

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  • N-Propargyl-1 (R)-aminoindan, rasagiline, increases glial ...

    2004-5-1u2002·u2002N-Propargyl-l(R)-aminoindan, rasagiline, an anti-Parkinson drug, was found to increase the protein and mRNA levels of glial cell line-derived neurotrophic factor (GDNF) in human neuroblastoma SH-SY5Y cells, whereas an analogue without a propargyl residue, aminoindan, did not.GDNF is known to protect dopaminergic neurons in animal and cellular models of Parkinson's disease, and the …

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  • A convenient protocol for establishing a human cell ...

    2018-7-18u2002·u2002The optic nerve is severed at the nerve head to detach the retina. The retinae are pooled in KCl buffer (0.3M KCl, 10mM HEPES, 0.5mM CaCl2, 1mM MgCl2; pH 7.0) with 48% sucrose (w/v) and agitated vigorously for 2 minutes on a rotation mixer after which the solution is …

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  • Animal‐cell culture media: History, characteristics, and ...

    2017-3-21u2002·u2002Furthermore, serum alters the conditions at a culture substratum, allowing the adherent cells to readily proliferate there. Fetal bovine serum (FBS) is the most popular and widely applicable serum today. Other kinds of serum are used in certain situations, including calf serum (CS) and horse serum.

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  • N-Propargyl-1 (R)-aminoindan, rasagiline, increases glial ...

    2004-5-1u2002·u2002N-Propargyl-l(R)-aminoindan, rasagiline, an anti-Parkinson drug, was found to increase the protein and mRNA levels of glial cell line-derived neurotrophic factor (GDNF) in human neuroblastoma SH-SY5Y cells, whereas an analogue without a propargyl residue, aminoindan, did not.GDNF is known to protect dopaminergic neurons in animal and cellular models of Parkinson's disease, and the …

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  • Co-ultramicronized Palmitoylethanolamide/Luteolin

    2015-11-18u2002·u2002Oligodendrocytes have limited ability to repair the damage to themselves or to other nerve cells, as seen in demyelinating diseases like multiple sclerosis. An important strategy may be to replace ...

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  • A convenient protocol for establishing a human cell ...

    2018-7-18u2002·u2002The optic nerve is severed at the nerve head to detach the retina. The retinae are pooled in KCl buffer (0.3M KCl, 10mM HEPES, 0.5mM CaCl2, 1mM MgCl2; pH 7.0) with 48% sucrose (w/v) and agitated vigorously for 2 minutes on a rotation mixer after which the solution is …

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    2021-9-29u2002·u2002Outstanding Paper Awards 2021. The Spine Journal and NASS created the Outstanding Paper Awards in 1989 to recognize excellence in unpublished research in spine care. The deadline has passed for submissions, but we look forward to announcing the 2021 award winners at the NASS conference in Boston, September 29-October 2.. To be the first to hear the news, please join us at the …

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    2017-3-21u2002·u20021. Introduction. The influence of cell culture technology on human society has been immeasurable. Progress in biology in recent years, for example, has depended heavily on cell culture technology. 1 In addition, cell culture‐based practical technologies have been developed in various areas, including the assessment of the efficacy and toxicity of new drugs, manufacture of …

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    2018-2-12u2002·u2002Neural stem cells (NSCs) are characterized by the ability of self‑renewal and capacity to proliferate and produce new nervous tissue. NSCs are capable of differentiating to three lineages of neural cells, including neurons, oligodendrocytes and astrocytes. Furthermore, hippocampal NSCs transplantation can improve the neurological deficits associated with expression of cytokines.

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    Rotavirus is a major pathogen responsible for diarrheal disease in calves, resulting in loss of productivity and economy of farmers. However, various facets of diarrheal disease caused by rotavirus in calves in the world are inadequately understood, considering that diarrheal disease caused by rotavirus is a vital health problem in calves that interrupts production benefits with …

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    2019-3-13u2002·u2002Hirschsprung's disease (HSCR) is a common congenital malformation of the enteric nervous system (ENS). During fetal development, ganglion cells of the ENS are derived from neural crest cells that migrate to the bowel. These cells reside principally in two ganglionated plexus: 1) The myenteric plexus, extending from the esophagus to the anus, and 2) submucous plexus, …

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    2021-9-29u2002·u2002Outstanding Paper Awards 2021. The Spine Journal and NASS created the Outstanding Paper Awards in 1989 to recognize excellence in unpublished research in spine care. The deadline has passed for submissions, but we look forward to announcing the 2021 award winners at the NASS conference in Boston, September 29-October 2.. To be the first to hear the news, please …

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    2016-3-14u2002·u2002Serum: Serum is a complex mixture of proteins, source of minerals, lipids, hormones, and growth and adhesion factors. Fetal bovine serum (FBS) and newborn calf serum (NCS) are most common. For more specific cultures human, horse or rabbit sera are used [52, 73] Antibiotics and antimycotic solutions

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