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  • Effect of oxidative stress on expression and function of ...

    2017-10-15u2002·u2002101 William's E medium (Invitrogen, Grand Island, NY) supplemented with 10% fetal bovine serum 102 (Thermo Fisher Scientific K.K.) and 100 IU/mL penicillin-100 μg/mL streptomycin (Sigma 103 Aldrich Japan) and left to attach for 6 h in an incubator at 37°C under 5% CO 2-95% air. 104 105 2.3. Treatment of HLE cells and rat primary hepatocytes ...

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  • Effects of Dehydroepiandrosterone on Gluconeogenic

    HepG2 cells obtained from Riken (Tsukuba, Japan) were maintained in Dulbecco's modified Eagle's medium (DMEM) containing 10% bovine fetal serum (FBS) at 37°C in 5% CO 2. To investigate the effect of each hormone, the cells were incubated for 24 h under the conditions indicated in the figure legends. Assay of G6Pase activity

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  • The polypeptide OP3-4 induced osteogenic differentiation ...

    The polypeptide OP 3-4 is known as a polypeptide that imitating osteoprotegerin, and its sequence is based on residues 113-122 of the human osteoprotegerin sequence (Cheng et al., 2004; Shin et al., 2008; Ta et al., 2010). OP 3-4 is designed based on osteoprotegerin's third cysteine-rich domain.

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  • A novel immortalized hepatocyte-like cell line (imHC ...

    2018-1-25u2002·u2002Characterization of immortalized hepatocyte-like cells (imHCs). imHCs were maintained in DMEM/F12 medium containing 10% fetal bovine serum. After reaching confluence, cells exhibited a typical hepatocyte morphology, including a polygonal shape, granulated cytoplasm, and large nucleus (a).Hepatocyte phenotypes were evaluated via immunofluorescence staining for the following major …

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  • Frontiers

    Colorectal cancer is a major cause of death worldwide, and the identification of new diagnostic and prognostic biomarkers is crucial to develop new strategies to avoid colorectal cancer-related deaths. Small nuclear ribonucleoprotein polypeptide N (SNRPN) is an imprinted gene that plays an important role in various neurodevelopmental disabilities.

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  • Chronic glucose-dependent insulinotropic polypeptide ...

    2020-10-5u2002·u2002Antagonism or agonism of the glucose-dependent insulinotropic polypeptide (GIP) receptor (GIPR) prevents weight gain and leads to dramatic weight loss in …

    Get Price
  • BAM8-22 and its receptor MRGPRX1 may attribute to ...

    2019-7-26u2002·u2002Pruritus is an unexpected symptom observed in cholestasis and its mechanism is still unclear. Here, we show that bovine adrenal medulla (BAM) 8–22, an …

    Get Price
  • Plasminogen promotes macrophage phagocytosis in mice ...

    2014-7-31u2002·u2002The mice used in experiments were 8 to10 weeks of age. J774A.1 cells, a murine macrophage-like cell line, were obtained from ATCC and maintained in DMEM containing 10% fetal bovine serum, 4 mM l-glutamine, 1.5 g/L sodium bicarbonate, 4.5g/L glucose and 1mM sodium pyruvate. For experiments, the J774A.1 cells were cultured in DMEM containing 1% ...

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  • DNA Methylation Profiles of Organic Anion Transporting ...

    2010-2-4u2002·u2002Purpose Multispecific organic anion transporter, OATP1B3/SLCO1B3, is expressed in several cancer cell lines as well as tumor tissues, and its expression sensitizes the cells to some anti-cancer agents. The present study was aimed to characterize the DNA methylation profiles around the transcriptional start site (TSS) of OATP1B3 and correlate them with the mRNA expression in cancer …

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  • Critical Factors in the Assessment of Cholestatic Liver ...

    2021-9-19u2002·u2002Hepatocyte seeding and culture medium. William's medium E containing 10 % fetal bovine serum (FBS), suitable antibiotics and 2 ng/mL insulin. Media should be warmed to 37 °C before use (see Note 1). Phosphate-buffered saline (PBS). 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 ·2H 2 O, 1.8 mM KH 2 PO 4 in deionized water. Adjust to pH 7.4 and ...

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  • Myricetin supplementation decreases hepatic lipid ...

    2021-8-31u2002·u2002HepG2 cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum and antibiotics in 75 cm 2 tissue culture flasks in a humidified atmosphere containing 5% CO 2 at 37°C. The medium was routinely changed every 3 days, and the cells were passaged by trypsinization before reaching ...

    Get Price
  • Pancreatic polypeptide inhibits somatostatin secretion ...

    2014-7-11u2002·u20021. Introduction. Pancreatic polypeptide (PP), gut derived peptide YY (PYY) and the neuronal derived peptide neuropeptide Y (NPY) belong to a family of structurally related peptides which have functions in both neural and endocrine signaling [1,2].Relatively little is known about the functional significance of PP, a 36–amino acid peptide secreted by PP (or F) cells of the islets of …

    Get Price
  • Transfer of the Cholera Toxin A1 Polypeptide from the ...

    DNA was introduced into the cells by a 3-h incubation with 1 μg of DNA and 5 μl of Lipofectamine as per the manufacturer's instructions (Invitrogen), and experiments were conducted at ~48 h posttransfection. Cells were maintained at 37°C and 5% CO 2 under humidified conditions in Ham's F-12 medium supplemented with 10% fetal bovine serum.

    Get Price
  • Bifunctional fused polypeptide inhibits the growth and ...

    2015-10-16u2002·u2002Introduction. Breast cancer is the second most common cancer in the world, and it is the most common cancer among women with an estimated 1.67 million new cancer cases diagnosed in 2012,1,2 and breast cancer ranks as the fifth cause of death from cancer overall, with a 522,000 deaths globally in 2012.1,2 Currently, the chemotherapy for breast cancer is often accompanied …

    Get Price
  • Pancreatic polypeptide inhibits somatostatin secretion ...

    2014-8-25u2002·u2002Pancreatic polypeptide (PP) is a major agonist for neuropeptide Y4 receptors (NPY4R). While NPY4R has been identified in various tissues, the cells on which it is expressed and its function in those cells has not been clearly delineated. Here we report that NPY4R is present in all somatostatin-containing cells of tissues that we tested ...

    Get Price
  • Chronic glucose-dependent insulinotropic polypeptide ...

    2020-10-5u2002·u2002Antagonism or agonism of the glucose-dependent insulinotropic polypeptide (GIP) receptor (GIPR) prevents weight gain and leads to dramatic weight loss in combination with glucagon-like peptide-1 ...

    Get Price
  • BAM8-22 and its receptor MRGPRX1 may attribute to ...

    2019-7-26u2002·u2002Pruritus is an unexpected symptom observed in cholestasis and its mechanism is still unclear. Here, we show that bovine adrenal medulla (BAM) 8–22, an endogenous itch-inducing peptide, could be ...

    Get Price
  • The polypeptide OP3-4 induced osteogenic differentiation ...

    The polypeptide OP 3-4 is known as a polypeptide that imitating osteoprotegerin, and its sequence is based on residues 113-122 of the human osteoprotegerin sequence (Cheng et al., 2004; Shin et al., 2008; Ta et al., 2010). OP 3-4 is designed based on …

    Get Price
  • Critical Factors in the Assessment of Cholestatic Liver ...

    2021-9-19u2002·u2002Hepatocyte seeding and culture medium. William's medium E containing 10 % fetal bovine serum (FBS), suitable antibiotics and 2 ng/mL insulin. Media should be warmed to 37 °C before use (see Note 1). Phosphate-buffered saline (PBS). 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 ·2H 2 O, 1.8 mM KH 2 PO 4 in deionized water. Adjust to pH 7.4 and ...

    Get Price
  • Myricetin supplementation decreases hepatic lipid ...

    2021-8-31u2002·u2002HepG2 cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum and antibiotics in 75 cm 2 tissue culture flasks in a humidified atmosphere containing 5% CO 2 at 37°C. The medium was routinely changed every 3 days, and the cells were passaged by trypsinization before reaching ...

    Get Price
  • Pancreatic polypeptide inhibits somatostatin secretion ...

    2014-7-11u2002·u20021. Introduction. Pancreatic polypeptide (PP), gut derived peptide YY (PYY) and the neuronal derived peptide neuropeptide Y (NPY) belong to a family of structurally related peptides which have functions in both neural and endocrine signaling [1,2].Relatively little is known about the functional significance of PP, a 36–amino acid peptide secreted by PP (or F) cells of the islets of Langerhans ...

    Get Price
  • Transfer of the Cholera Toxin A1 Polypeptide from the ...

    DNA was introduced into the cells by a 3-h incubation with 1 μg of DNA and 5 μl of Lipofectamine as per the manufacturer's instructions (Invitrogen), and experiments were conducted at ~48 h posttransfection. Cells were maintained at 37°C and 5% CO 2 under humidified conditions in Ham's F-12 medium supplemented with 10% fetal bovine serum.

    Get Price
  • Bifunctional fused polypeptide inhibits the growth and ...

    2015-10-16u2002·u2002Introduction. Breast cancer is the second most common cancer in the world, and it is the most common cancer among women with an estimated 1.67 million new cancer cases diagnosed in 2012,1,2 and breast cancer ranks as the fifth cause of death from cancer overall, with a 522,000 deaths globally in 2012.1,2 Currently, the chemotherapy for breast cancer is often accompanied with side …

    Get Price
  • Pancreatic polypeptide inhibits somatostatin secretion ...

    2014-8-25u2002·u2002Pancreatic polypeptide (PP) is a major agonist for neuropeptide Y4 receptors (NPY4R). While NPY4R has been identified in various tissues, the cells on which it is expressed and its function in those cells has not been clearly delineated. Here we report that NPY4R is present in all somatostatin-containing cells of tissues that we tested ...

    Get Price
  • Chronic glucose-dependent insulinotropic polypeptide ...

    2020-10-5u2002·u2002Antagonism or agonism of the glucose-dependent insulinotropic polypeptide (GIP) receptor (GIPR) prevents weight gain and leads to dramatic weight loss in …

    Get Price
  • BAM8-22 and its receptor MRGPRX1 may attribute to ...

    2019-7-26u2002·u2002Pruritus is an unexpected symptom observed in cholestasis and its mechanism is still unclear. Here, we show that bovine adrenal medulla (BAM) 8–22, an …

    Get Price
  • The polypeptide OP3-4 induced osteogenic differentiation ...

    The polypeptide OP 3-4 is known as a polypeptide that imitating osteoprotegerin, and its sequence is based on residues 113-122 of the human osteoprotegerin sequence (Cheng et al., 2004; Shin et al., 2008; Ta et al., 2010). OP 3-4 is designed based on osteoprotegerin's third cysteine-rich domain.

    Get Price
  • Critical Factors in the Assessment of Cholestatic Liver ...

    2021-9-19u2002·u2002Hepatocyte seeding and culture medium. William's medium E containing 10 % fetal bovine serum (FBS), suitable antibiotics and 2 ng/mL insulin. Media should be warmed to 37 °C before use (see Note 1). Phosphate-buffered saline (PBS). 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 ·2H 2 O, 1.8 mM KH 2 PO 4 in deionized water. Adjust to pH 7.4 and ...

    Get Price
  • Myricetin supplementation decreases hepatic lipid ...

    2021-8-31u2002·u2002HepG2 cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum and antibiotics in 75 cm 2 tissue culture flasks in a humidified atmosphere containing 5% CO 2 at 37°C. The medium was routinely changed every 3 days, and the cells were passaged by trypsinization before reaching ...

    Get Price
  • Pancreatic polypeptide inhibits somatostatin secretion ...

    2014-7-11u2002·u20021. Introduction. Pancreatic polypeptide (PP), gut derived peptide YY (PYY) and the neuronal derived peptide neuropeptide Y (NPY) belong to a family of structurally related peptides which have functions in both neural and endocrine signaling [1,2].Relatively little is known about the functional significance of PP, a 36–amino acid peptide secreted by PP (or F) cells of the islets of …

    Get Price
  • Transfer of the Cholera Toxin A1 Polypeptide from the ...

    DNA was introduced into the cells by a 3-h incubation with 1 μg of DNA and 5 μl of Lipofectamine as per the manufacturer's instructions (Invitrogen), and experiments were conducted at ~48 h posttransfection. Cells were maintained at 37°C and 5% CO 2 under humidified conditions in Ham's F-12 medium supplemented with 10% fetal bovine serum.

    Get Price
  • Bifunctional fused polypeptide inhibits the growth and ...

    2015-10-16u2002·u2002Introduction. Breast cancer is the second most common cancer in the world, and it is the most common cancer among women with an estimated 1.67 million new cancer cases diagnosed in 2012,1,2 and breast cancer ranks as the fifth cause of death from cancer overall, with a 522,000 deaths globally in 2012.1,2 Currently, the chemotherapy for breast cancer is often accompanied …

    Get Price
  • Pancreatic polypeptide inhibits somatostatin secretion ...

    2014-8-25u2002·u2002Pancreatic polypeptide (PP) is a major agonist for neuropeptide Y4 receptors (NPY4R). While NPY4R has been identified in various tissues, the cells on which it is expressed and its function in those cells has not been clearly delineated. Here we report that NPY4R is present in all somatostatin-containing cells of tissues that we tested ...

    Get Price
  • Chronic glucose-dependent insulinotropic polypeptide ...

    2020-10-5u2002·u2002Antagonism or agonism of the glucose-dependent insulinotropic polypeptide (GIP) receptor (GIPR) prevents weight gain and leads to dramatic weight loss in combination with glucagon-like peptide-1 ...

    Get Price
  • BAM8-22 and its receptor MRGPRX1 may attribute to ...

    2019-7-26u2002·u2002Pruritus is an unexpected symptom observed in cholestasis and its mechanism is still unclear. Here, we show that bovine adrenal medulla (BAM) 8–22, an endogenous itch-inducing peptide, could be ...

    Get Price
  • The polypeptide OP3-4 induced osteogenic differentiation ...

    The polypeptide OP 3-4 is known as a polypeptide that imitating osteoprotegerin, and its sequence is based on residues 113-122 of the human osteoprotegerin sequence (Cheng et al., 2004; Shin et al., 2008; Ta et al., 2010). OP 3-4 is designed based on …

    Get Price
  • Critical Factors in the Assessment of Cholestatic Liver ...

    2021-9-19u2002·u2002Hepatocyte seeding and culture medium. William's medium E containing 10 % fetal bovine serum (FBS), suitable antibiotics and 2 ng/mL insulin. Media should be warmed to 37 °C before use (see Note 1). Phosphate-buffered saline (PBS). 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 ·2H 2 O, 1.8 mM KH 2 PO 4 in deionized water. Adjust to pH 7.4 and ...

    Get Price
  • Myricetin supplementation decreases hepatic lipid ...

    2021-8-31u2002·u2002HepG2 cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum and antibiotics in 75 cm 2 tissue culture flasks in a humidified atmosphere containing 5% CO 2 at 37°C. The medium was routinely changed every 3 days, and the cells were passaged by trypsinization before reaching ...

    Get Price
  • Pancreatic polypeptide inhibits somatostatin secretion ...

    2014-7-11u2002·u20021. Introduction. Pancreatic polypeptide (PP), gut derived peptide YY (PYY) and the neuronal derived peptide neuropeptide Y (NPY) belong to a family of structurally related peptides which have functions in both neural and endocrine signaling [1,2].Relatively little is known about the functional significance of PP, a 36–amino acid peptide secreted by PP (or F) cells of the islets of Langerhans ...

    Get Price
  • Transfer of the Cholera Toxin A1 Polypeptide from the ...

    DNA was introduced into the cells by a 3-h incubation with 1 μg of DNA and 5 μl of Lipofectamine as per the manufacturer's instructions (Invitrogen), and experiments were conducted at ~48 h posttransfection. Cells were maintained at 37°C and 5% CO 2 under humidified conditions in Ham's F-12 medium supplemented with 10% fetal bovine serum.

    Get Price
  • Bifunctional fused polypeptide inhibits the growth and ...

    2015-10-16u2002·u2002Introduction. Breast cancer is the second most common cancer in the world, and it is the most common cancer among women with an estimated 1.67 million new cancer cases diagnosed in 2012,1,2 and breast cancer ranks as the fifth cause of death from cancer overall, with a 522,000 deaths globally in 2012.1,2 Currently, the chemotherapy for breast cancer is often accompanied with side …

    Get Price
  • Pancreatic polypeptide inhibits somatostatin secretion ...

    2014-8-25u2002·u2002Pancreatic polypeptide (PP) is a major agonist for neuropeptide Y4 receptors (NPY4R). While NPY4R has been identified in various tissues, the cells on which it is expressed and its function in those cells has not been clearly delineated. Here we report that NPY4R is present in all somatostatin-containing cells of tissues that we tested ...

    Get Price
  • Chronic glucose-dependent insulinotropic polypeptide ...

    2020-10-5u2002·u2002Antagonism or agonism of the glucose-dependent insulinotropic polypeptide (GIP) receptor (GIPR) prevents weight gain and leads to dramatic weight loss in …

    Get Price
  • BAM8-22 and its receptor MRGPRX1 may attribute to ...

    2019-7-26u2002·u2002Pruritus is an unexpected symptom observed in cholestasis and its mechanism is still unclear. Here, we show that bovine adrenal medulla (BAM) 8–22, an …

    Get Price
  • The polypeptide OP3-4 induced osteogenic differentiation ...

    The polypeptide OP 3-4 is known as a polypeptide that imitating osteoprotegerin, and its sequence is based on residues 113-122 of the human osteoprotegerin sequence (Cheng et al., 2004; Shin et al., 2008; Ta et al., 2010). OP 3-4 is designed based on osteoprotegerin's third cysteine-rich domain.

    Get Price
  • Critical Factors in the Assessment of Cholestatic Liver ...

    2021-9-19u2002·u2002Hepatocyte seeding and culture medium. William's medium E containing 10 % fetal bovine serum (FBS), suitable antibiotics and 2 ng/mL insulin. Media should be warmed to 37 °C before use (see Note 1). Phosphate-buffered saline (PBS). 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 ·2H 2 O, 1.8 mM KH 2 PO 4 in deionized water. Adjust to pH 7.4 and ...

    Get Price
  • Myricetin supplementation decreases hepatic lipid ...

    2021-8-31u2002·u2002HepG2 cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum and antibiotics in 75 cm 2 tissue culture flasks in a humidified atmosphere containing 5% CO 2 at 37°C. The medium was routinely changed every 3 days, and the cells were passaged by trypsinization before reaching ...

    Get Price
  • Pancreatic polypeptide inhibits somatostatin secretion ...

    2014-7-11u2002·u20021. Introduction. Pancreatic polypeptide (PP), gut derived peptide YY (PYY) and the neuronal derived peptide neuropeptide Y (NPY) belong to a family of structurally related peptides which have functions in both neural and endocrine signaling [1,2].Relatively little is known about the functional significance of PP, a 36–amino acid peptide secreted by PP (or F) cells of the …

    Get Price
  • Transfer of the Cholera Toxin A1 Polypeptide from the ...

    DNA was introduced into the cells by a 3-h incubation with 1 μg of DNA and 5 μl of Lipofectamine as per the manufacturer's instructions (Invitrogen), and experiments were conducted at ~48 h posttransfection. Cells were maintained at 37°C and 5% CO 2 under humidified conditions in Ham's F-12 medium supplemented with 10% fetal bovine serum.

    Get Price
  • Bifunctional fused polypeptide inhibits the growth and ...

    2015-10-16u2002·u2002Introduction. Breast cancer is the second most common cancer in the world, and it is the most common cancer among women with an estimated 1.67 million new cancer cases diagnosed in 2012,1,2 and breast cancer ranks as the fifth cause of death from cancer overall, with a 522,000 deaths globally in 2012.1,2 Currently, the chemotherapy for breast cancer is …

    Get Price
  • Pancreatic polypeptide inhibits somatostatin secretion ...

    2014-8-25u2002·u2002Pancreatic polypeptide (PP) is a major agonist for neuropeptide Y4 receptors (NPY4R). While NPY4R has been identified in various tissues, the cells on which it is expressed and its function in those cells has not been clearly delineated. Here we report that NPY4R is present in all somatostatin-containing cells of tissues that we tested ...

    Get Price
  • Chronic glucose-dependent insulinotropic polypeptide ...

    2020-10-5u2002·u2002Antagonism or agonism of the glucose-dependent insulinotropic polypeptide (GIP) receptor (GIPR) prevents weight gain and leads to dramatic weight loss in combination with glucagon-like peptide-1 ...

    Get Price
  • BAM8-22 and its receptor MRGPRX1 may attribute to ...

    2019-7-26u2002·u2002Pruritus is an unexpected symptom observed in cholestasis and its mechanism is still unclear. Here, we show that bovine adrenal medulla (BAM) 8–22, an endogenous itch-inducing peptide, could be ...

    Get Price
  • The polypeptide OP3-4 induced osteogenic differentiation ...

    The polypeptide OP 3-4 is known as a polypeptide that imitating osteoprotegerin, and its sequence is based on residues 113-122 of the human osteoprotegerin sequence (Cheng et al., 2004; Shin et al., 2008; Ta et al., 2010). OP 3-4 is designed based on osteoprotegerin's third cysteine-rich domain.

    Get Price