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2017-10-15u2002·u2002101 William's E medium (Invitrogen, Grand Island, NY) supplemented with 10% fetal bovine serum 102 (Thermo Fisher Scientific K.K.) and 100 IU/mL penicillin-100 μg/mL streptomycin (Sigma 103 Aldrich Japan) and left to attach for 6 h in an incubator at 37°C under 5% CO 2-95% air. 104 105 2.3. Treatment of HLE cells and rat primary hepatocytes ...
Get PriceHepG2 cells obtained from Riken (Tsukuba, Japan) were maintained in Dulbecco's modified Eagle's medium (DMEM) containing 10% bovine fetal serum (FBS) at 37°C in 5% CO 2. To investigate the effect of each hormone, the cells were incubated for 24 h under the conditions indicated in the figure legends. Assay of G6Pase activity
Get PriceThe polypeptide OP 3-4 is known as a polypeptide that imitating osteoprotegerin, and its sequence is based on residues 113-122 of the human osteoprotegerin sequence (Cheng et al., 2004; Shin et al., 2008; Ta et al., 2010). OP 3-4 is designed based on osteoprotegerin's third cysteine-rich domain.
Get Price2018-1-25u2002·u2002Characterization of immortalized hepatocyte-like cells (imHCs). imHCs were maintained in DMEM/F12 medium containing 10% fetal bovine serum. After reaching confluence, cells exhibited a typical hepatocyte morphology, including a polygonal shape, granulated cytoplasm, and large nucleus (a).Hepatocyte phenotypes were evaluated via immunofluorescence staining for the following major …
Get PriceColorectal cancer is a major cause of death worldwide, and the identification of new diagnostic and prognostic biomarkers is crucial to develop new strategies to avoid colorectal cancer-related deaths. Small nuclear ribonucleoprotein polypeptide N (SNRPN) is an imprinted gene that plays an important role in various neurodevelopmental disabilities.
Get Price2020-10-5u2002·u2002Antagonism or agonism of the glucose-dependent insulinotropic polypeptide (GIP) receptor (GIPR) prevents weight gain and leads to dramatic weight loss in …
Get Price2019-7-26u2002·u2002Pruritus is an unexpected symptom observed in cholestasis and its mechanism is still unclear. Here, we show that bovine adrenal medulla (BAM) 8–22, an …
Get Price2014-7-31u2002·u2002The mice used in experiments were 8 to10 weeks of age. J774A.1 cells, a murine macrophage-like cell line, were obtained from ATCC and maintained in DMEM containing 10% fetal bovine serum, 4 mM l-glutamine, 1.5 g/L sodium bicarbonate, 4.5g/L glucose and 1mM sodium pyruvate. For experiments, the J774A.1 cells were cultured in DMEM containing 1% ...
Get Price2010-2-4u2002·u2002Purpose Multispecific organic anion transporter, OATP1B3/SLCO1B3, is expressed in several cancer cell lines as well as tumor tissues, and its expression sensitizes the cells to some anti-cancer agents. The present study was aimed to characterize the DNA methylation profiles around the transcriptional start site (TSS) of OATP1B3 and correlate them with the mRNA expression in cancer …
Get Price2021-9-19u2002·u2002Hepatocyte seeding and culture medium. William's medium E containing 10 % fetal bovine serum (FBS), suitable antibiotics and 2 ng/mL insulin. Media should be warmed to 37 °C before use (see Note 1). Phosphate-buffered saline (PBS). 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 ·2H 2 O, 1.8 mM KH 2 PO 4 in deionized water. Adjust to pH 7.4 and ...
Get Price2021-8-31u2002·u2002HepG2 cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum and antibiotics in 75 cm 2 tissue culture flasks in a humidified atmosphere containing 5% CO 2 at 37°C. The medium was routinely changed every 3 days, and the cells were passaged by trypsinization before reaching ...
Get Price2014-7-11u2002·u20021. Introduction. Pancreatic polypeptide (PP), gut derived peptide YY (PYY) and the neuronal derived peptide neuropeptide Y (NPY) belong to a family of structurally related peptides which have functions in both neural and endocrine signaling [1,2].Relatively little is known about the functional significance of PP, a 36–amino acid peptide secreted by PP (or F) cells of the islets of …
Get PriceDNA was introduced into the cells by a 3-h incubation with 1 μg of DNA and 5 μl of Lipofectamine as per the manufacturer's instructions (Invitrogen), and experiments were conducted at ~48 h posttransfection. Cells were maintained at 37°C and 5% CO 2 under humidified conditions in Ham's F-12 medium supplemented with 10% fetal bovine serum.
Get Price2015-10-16u2002·u2002Introduction. Breast cancer is the second most common cancer in the world, and it is the most common cancer among women with an estimated 1.67 million new cancer cases diagnosed in 2012,1,2 and breast cancer ranks as the fifth cause of death from cancer overall, with a 522,000 deaths globally in 2012.1,2 Currently, the chemotherapy for breast cancer is often accompanied …
Get Price2014-8-25u2002·u2002Pancreatic polypeptide (PP) is a major agonist for neuropeptide Y4 receptors (NPY4R). While NPY4R has been identified in various tissues, the cells on which it is expressed and its function in those cells has not been clearly delineated. Here we report that NPY4R is present in all somatostatin-containing cells of tissues that we tested ...
Get Price2020-10-5u2002·u2002Antagonism or agonism of the glucose-dependent insulinotropic polypeptide (GIP) receptor (GIPR) prevents weight gain and leads to dramatic weight loss in combination with glucagon-like peptide-1 ...
Get Price2019-7-26u2002·u2002Pruritus is an unexpected symptom observed in cholestasis and its mechanism is still unclear. Here, we show that bovine adrenal medulla (BAM) 8–22, an endogenous itch-inducing peptide, could be ...
Get PriceThe polypeptide OP 3-4 is known as a polypeptide that imitating osteoprotegerin, and its sequence is based on residues 113-122 of the human osteoprotegerin sequence (Cheng et al., 2004; Shin et al., 2008; Ta et al., 2010). OP 3-4 is designed based on …
Get Price2021-9-19u2002·u2002Hepatocyte seeding and culture medium. William's medium E containing 10 % fetal bovine serum (FBS), suitable antibiotics and 2 ng/mL insulin. Media should be warmed to 37 °C before use (see Note 1). Phosphate-buffered saline (PBS). 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 ·2H 2 O, 1.8 mM KH 2 PO 4 in deionized water. Adjust to pH 7.4 and ...
Get Price2021-8-31u2002·u2002HepG2 cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum and antibiotics in 75 cm 2 tissue culture flasks in a humidified atmosphere containing 5% CO 2 at 37°C. The medium was routinely changed every 3 days, and the cells were passaged by trypsinization before reaching ...
Get Price2014-7-11u2002·u20021. Introduction. Pancreatic polypeptide (PP), gut derived peptide YY (PYY) and the neuronal derived peptide neuropeptide Y (NPY) belong to a family of structurally related peptides which have functions in both neural and endocrine signaling [1,2].Relatively little is known about the functional significance of PP, a 36–amino acid peptide secreted by PP (or F) cells of the islets of Langerhans ...
Get PriceDNA was introduced into the cells by a 3-h incubation with 1 μg of DNA and 5 μl of Lipofectamine as per the manufacturer's instructions (Invitrogen), and experiments were conducted at ~48 h posttransfection. Cells were maintained at 37°C and 5% CO 2 under humidified conditions in Ham's F-12 medium supplemented with 10% fetal bovine serum.
Get Price2015-10-16u2002·u2002Introduction. Breast cancer is the second most common cancer in the world, and it is the most common cancer among women with an estimated 1.67 million new cancer cases diagnosed in 2012,1,2 and breast cancer ranks as the fifth cause of death from cancer overall, with a 522,000 deaths globally in 2012.1,2 Currently, the chemotherapy for breast cancer is often accompanied with side …
Get Price2014-8-25u2002·u2002Pancreatic polypeptide (PP) is a major agonist for neuropeptide Y4 receptors (NPY4R). While NPY4R has been identified in various tissues, the cells on which it is expressed and its function in those cells has not been clearly delineated. Here we report that NPY4R is present in all somatostatin-containing cells of tissues that we tested ...
Get Price2020-10-5u2002·u2002Antagonism or agonism of the glucose-dependent insulinotropic polypeptide (GIP) receptor (GIPR) prevents weight gain and leads to dramatic weight loss in …
Get Price2019-7-26u2002·u2002Pruritus is an unexpected symptom observed in cholestasis and its mechanism is still unclear. Here, we show that bovine adrenal medulla (BAM) 8–22, an …
Get PriceThe polypeptide OP 3-4 is known as a polypeptide that imitating osteoprotegerin, and its sequence is based on residues 113-122 of the human osteoprotegerin sequence (Cheng et al., 2004; Shin et al., 2008; Ta et al., 2010). OP 3-4 is designed based on osteoprotegerin's third cysteine-rich domain.
Get Price2021-9-19u2002·u2002Hepatocyte seeding and culture medium. William's medium E containing 10 % fetal bovine serum (FBS), suitable antibiotics and 2 ng/mL insulin. Media should be warmed to 37 °C before use (see Note 1). Phosphate-buffered saline (PBS). 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 ·2H 2 O, 1.8 mM KH 2 PO 4 in deionized water. Adjust to pH 7.4 and ...
Get Price2021-8-31u2002·u2002HepG2 cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum and antibiotics in 75 cm 2 tissue culture flasks in a humidified atmosphere containing 5% CO 2 at 37°C. The medium was routinely changed every 3 days, and the cells were passaged by trypsinization before reaching ...
Get Price2014-7-11u2002·u20021. Introduction. Pancreatic polypeptide (PP), gut derived peptide YY (PYY) and the neuronal derived peptide neuropeptide Y (NPY) belong to a family of structurally related peptides which have functions in both neural and endocrine signaling [1,2].Relatively little is known about the functional significance of PP, a 36–amino acid peptide secreted by PP (or F) cells of the islets of …
Get PriceDNA was introduced into the cells by a 3-h incubation with 1 μg of DNA and 5 μl of Lipofectamine as per the manufacturer's instructions (Invitrogen), and experiments were conducted at ~48 h posttransfection. Cells were maintained at 37°C and 5% CO 2 under humidified conditions in Ham's F-12 medium supplemented with 10% fetal bovine serum.
Get Price2015-10-16u2002·u2002Introduction. Breast cancer is the second most common cancer in the world, and it is the most common cancer among women with an estimated 1.67 million new cancer cases diagnosed in 2012,1,2 and breast cancer ranks as the fifth cause of death from cancer overall, with a 522,000 deaths globally in 2012.1,2 Currently, the chemotherapy for breast cancer is often accompanied …
Get Price2014-8-25u2002·u2002Pancreatic polypeptide (PP) is a major agonist for neuropeptide Y4 receptors (NPY4R). While NPY4R has been identified in various tissues, the cells on which it is expressed and its function in those cells has not been clearly delineated. Here we report that NPY4R is present in all somatostatin-containing cells of tissues that we tested ...
Get Price2020-10-5u2002·u2002Antagonism or agonism of the glucose-dependent insulinotropic polypeptide (GIP) receptor (GIPR) prevents weight gain and leads to dramatic weight loss in combination with glucagon-like peptide-1 ...
Get Price2019-7-26u2002·u2002Pruritus is an unexpected symptom observed in cholestasis and its mechanism is still unclear. Here, we show that bovine adrenal medulla (BAM) 8–22, an endogenous itch-inducing peptide, could be ...
Get PriceThe polypeptide OP 3-4 is known as a polypeptide that imitating osteoprotegerin, and its sequence is based on residues 113-122 of the human osteoprotegerin sequence (Cheng et al., 2004; Shin et al., 2008; Ta et al., 2010). OP 3-4 is designed based on …
Get Price2021-9-19u2002·u2002Hepatocyte seeding and culture medium. William's medium E containing 10 % fetal bovine serum (FBS), suitable antibiotics and 2 ng/mL insulin. Media should be warmed to 37 °C before use (see Note 1). Phosphate-buffered saline (PBS). 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 ·2H 2 O, 1.8 mM KH 2 PO 4 in deionized water. Adjust to pH 7.4 and ...
Get Price2021-8-31u2002·u2002HepG2 cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum and antibiotics in 75 cm 2 tissue culture flasks in a humidified atmosphere containing 5% CO 2 at 37°C. The medium was routinely changed every 3 days, and the cells were passaged by trypsinization before reaching ...
Get Price2014-7-11u2002·u20021. Introduction. Pancreatic polypeptide (PP), gut derived peptide YY (PYY) and the neuronal derived peptide neuropeptide Y (NPY) belong to a family of structurally related peptides which have functions in both neural and endocrine signaling [1,2].Relatively little is known about the functional significance of PP, a 36–amino acid peptide secreted by PP (or F) cells of the islets of Langerhans ...
Get PriceDNA was introduced into the cells by a 3-h incubation with 1 μg of DNA and 5 μl of Lipofectamine as per the manufacturer's instructions (Invitrogen), and experiments were conducted at ~48 h posttransfection. Cells were maintained at 37°C and 5% CO 2 under humidified conditions in Ham's F-12 medium supplemented with 10% fetal bovine serum.
Get Price2015-10-16u2002·u2002Introduction. Breast cancer is the second most common cancer in the world, and it is the most common cancer among women with an estimated 1.67 million new cancer cases diagnosed in 2012,1,2 and breast cancer ranks as the fifth cause of death from cancer overall, with a 522,000 deaths globally in 2012.1,2 Currently, the chemotherapy for breast cancer is often accompanied with side …
Get Price2014-8-25u2002·u2002Pancreatic polypeptide (PP) is a major agonist for neuropeptide Y4 receptors (NPY4R). While NPY4R has been identified in various tissues, the cells on which it is expressed and its function in those cells has not been clearly delineated. Here we report that NPY4R is present in all somatostatin-containing cells of tissues that we tested ...
Get Price2020-10-5u2002·u2002Antagonism or agonism of the glucose-dependent insulinotropic polypeptide (GIP) receptor (GIPR) prevents weight gain and leads to dramatic weight loss in …
Get Price2019-7-26u2002·u2002Pruritus is an unexpected symptom observed in cholestasis and its mechanism is still unclear. Here, we show that bovine adrenal medulla (BAM) 8–22, an …
Get PriceThe polypeptide OP 3-4 is known as a polypeptide that imitating osteoprotegerin, and its sequence is based on residues 113-122 of the human osteoprotegerin sequence (Cheng et al., 2004; Shin et al., 2008; Ta et al., 2010). OP 3-4 is designed based on osteoprotegerin's third cysteine-rich domain.
Get Price2021-9-19u2002·u2002Hepatocyte seeding and culture medium. William's medium E containing 10 % fetal bovine serum (FBS), suitable antibiotics and 2 ng/mL insulin. Media should be warmed to 37 °C before use (see Note 1). Phosphate-buffered saline (PBS). 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 ·2H 2 O, 1.8 mM KH 2 PO 4 in deionized water. Adjust to pH 7.4 and ...
Get Price2021-8-31u2002·u2002HepG2 cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum and antibiotics in 75 cm 2 tissue culture flasks in a humidified atmosphere containing 5% CO 2 at 37°C. The medium was routinely changed every 3 days, and the cells were passaged by trypsinization before reaching ...
Get Price2014-7-11u2002·u20021. Introduction. Pancreatic polypeptide (PP), gut derived peptide YY (PYY) and the neuronal derived peptide neuropeptide Y (NPY) belong to a family of structurally related peptides which have functions in both neural and endocrine signaling [1,2].Relatively little is known about the functional significance of PP, a 36–amino acid peptide secreted by PP (or F) cells of the …
Get PriceDNA was introduced into the cells by a 3-h incubation with 1 μg of DNA and 5 μl of Lipofectamine as per the manufacturer's instructions (Invitrogen), and experiments were conducted at ~48 h posttransfection. Cells were maintained at 37°C and 5% CO 2 under humidified conditions in Ham's F-12 medium supplemented with 10% fetal bovine serum.
Get Price2015-10-16u2002·u2002Introduction. Breast cancer is the second most common cancer in the world, and it is the most common cancer among women with an estimated 1.67 million new cancer cases diagnosed in 2012,1,2 and breast cancer ranks as the fifth cause of death from cancer overall, with a 522,000 deaths globally in 2012.1,2 Currently, the chemotherapy for breast cancer is …
Get Price2014-8-25u2002·u2002Pancreatic polypeptide (PP) is a major agonist for neuropeptide Y4 receptors (NPY4R). While NPY4R has been identified in various tissues, the cells on which it is expressed and its function in those cells has not been clearly delineated. Here we report that NPY4R is present in all somatostatin-containing cells of tissues that we tested ...
Get Price2020-10-5u2002·u2002Antagonism or agonism of the glucose-dependent insulinotropic polypeptide (GIP) receptor (GIPR) prevents weight gain and leads to dramatic weight loss in combination with glucagon-like peptide-1 ...
Get Price2019-7-26u2002·u2002Pruritus is an unexpected symptom observed in cholestasis and its mechanism is still unclear. Here, we show that bovine adrenal medulla (BAM) 8–22, an endogenous itch-inducing peptide, could be ...
Get PriceThe polypeptide OP 3-4 is known as a polypeptide that imitating osteoprotegerin, and its sequence is based on residues 113-122 of the human osteoprotegerin sequence (Cheng et al., 2004; Shin et al., 2008; Ta et al., 2010). OP 3-4 is designed based on osteoprotegerin's third cysteine-rich domain.
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